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Replacement of RNA hairpins by in vitro selected tetranucleotides
journal contribution
posted on 1993-01-01, 00:00 authored by Bernhard DichtlBernhard Dichtl, T Pan, A di Renzo, O UhlenbeckAn in vitro selection method based on the autolytic cleavage of yeast tRNAPhe by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucieotide sequences allow proper folding of the rest of the tRNA molecule, wheras others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNAPhe and the anticodon hairpin of E.coli tRNAPhe without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally non essential hairpins in any RNA.
History
Journal
Nucleic acids researchVolume
21Issue
3Pagination
531 - 535Publisher
Oxford University PressLocation
Oxford, EnglandPublisher DOI
ISSN
0305-1048eISSN
1362-4962Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1993, Oxford University PressUsage metrics
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