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Reporting evidence of resistance against severe metabolic stress in human lung carcinoma

journal contribution
posted on 2022-10-25, 22:38 authored by Maryam Nakhjavani, F H Shirazi
Many cancer patients suffer from failure in the treatment and cancer recurrence, usually with a more resistant character. It is because a tumor is a heterogeneous tissue and cells within a tumor are in different metabolic stages. The aim of this study was to investigate the effect of long-term metabolic stress on human lung cancer, A549 cell line. The cells were seeded at the density of 4000 cells/cm2. Upon reaching to plateau state, the cells were exposed to serum-free media for 6 days. At 24-hour intervals, the cells were tested for morphology, live cell count, cell cycle distribution, cell size, mitochondrial function and protein content. Also, after each day of starvation, starved cells were re-exposed to media containing 10% serum, for 24 hours. Then the above tests were performed on the cells. All experiments were performed triplicate. Results showed that, within the study period, live cell count was less than control cells. However, the cell count was kept at a plateau-like state with an increasing trend on the final day of the study. Up to 73.5% of the cells accumulated in the G1 phase. Upon releasing the cells, the G1 population decreased and the cells entered S and G2/M phases. However, cell size did not change significantly during the study. The mitochondrial function and protein content of the cells had an increasing trend in both starvation and release states. The results are suggestive of a highly resistant characteristic of this cell line when exposed to long-term serum-free metabolic stress.

History

Journal

Fabad Journal of Pharmaceutical Sciences

Volume

44

Pagination

89 - 99

ISSN

1300-4182

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