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Residual DNA on examination tools following use
journal contribution
posted on 2015-12-01, 00:00 authored by Bianca SzkutaBianca Szkuta, Michelle HarveyMichelle Harvey, K N Ballantyne, R A H van OorschotRecent studies observing the transfer of DNA via examination tools used within forensic laboratories (scissors, forceps and gloves) have highlighted the contamination risk of such implements if protocols following their use and replacement are not adhered to. Whilst these previous studies focus primarily on the transfer of biological substances to a substrate via high-risk vectors, this investigation considers the proportion of DNA that remains on the high-risk vectors following contact with the substrate. Dried blood or touch DNA was deposited on cotton or glass substrates to create mock exhibits. Following primary contact with the deposit, the vector similarly contacted a secondary DNA-free substrate. Combinations of singular and multiple contacts were applied. Immediately following contact with the secondary substrate, the vector was sampled in order to determine the proportion of DNA-containing material remaining on the vectors following contacts. Residual DNA was detected on the vectors in most instances, with the amount retained influenced by the vector, substrates and biological substance applied. The results demonstrate the potential for inter- and intra-exhibit contamination through further contacts.
History
Journal
Forensic science international: genetics supplement seriesVolume
5Pagination
495 - 497Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
1875-1768eISSN
1875-175XLanguage
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, ElsevierUsage metrics
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