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Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle
journal contribution
posted on 2007-06-01, 00:00 authored by Kirsten HowlettKirsten Howlett, K Sakamoto, Andrew GarnhamAndrew Garnham, David Cameron-Smith, Mark HargreavesA single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs after resistance exercise is unknown. Hyperinsulinemic-euglycemic clamps were performed on eight male subjects at rest and after a single bout and three repeated bouts of resistance exercise over 7 days. Skeletal muscle biopsies were taken before and after the clamp and immediately after a single exercise bout. Whole-body insulin action measured by glucose infusion rate decreased (P < 0.05) after a single exercise bout, whereas in response to repeated bouts of resistance exercise, the glucose infusion rate was similar to the rest trial. In skeletal muscle, Akt substrate of 160 kDa (AS160) phosphorylation, an Akt substrate implicated in the regulation of GLUT4 translocation, and its interaction with 14-3-3 was decreased (P < 0.05) only after a single exercise bout. Insulin increased (P < 0.05) phosphorylation of AS160 and its interaction with 14-3-3, but the insulin response was not influenced by resistance exercise. Phosphorylation of insulin receptor substrate-1 and Akt were similar to changes in AS160 phosphorylation after exercise and/or insulin. In conclusion, a single bout of resistance exercise impairs whole-body insulin action. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action.
History
Journal
DiabetesVolume
56Issue
6Pagination
1608 - 1614Publisher
American Diabetes AssociationLocation
New York, N.Y.Publisher DOI
ISSN
0012-1797eISSN
1939-327XLanguage
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2007, American Diabetes AssociationUsage metrics
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