hamilton-resistanceexercise-2017.pdf (10.38 MB)
Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle
journal contribution
posted on 2017-07-10, 00:00 authored by Zhe Song, Daniel R Moore, Nathan Hodson, Carl Ward, Jessica R Dent, Mary F O'Leary, Andrew M Shaw, Lee HamiltonLee Hamilton, Sovan Sarkar, Yann-Gaël Gangloff, Troy A Hornberger, Lawrence L Spriet, George J Heigenhauser, Andrew PhilpThe mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly co-localised in basal samples. Resistance exercise resulted in rapid translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance exercise led to the dissociation of TSC2 from Rheb and increased in the co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F (eIF3F) at the cell membrane post-exercise in both groups, with the response significantly greater at 1 h of recovery in the FED compared to CON. Collectively our data demonstrate that cellular trafficking of mTOR occurs in human muscle in response to an anabolic stimulus, events that appear to be primarily influenced by muscle contraction. The translocation and association of mTOR with positive regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA translational capacity after resistance exercise.
History
Journal
Scientific reportsVolume
7Article number
5028Pagination
1 - 14Publisher
Nature Publishing GroupLocation
London, Eng.Publisher DOI
Link to full text
eISSN
2045-2322Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2017, The AuthorsUsage metrics
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Cell biologyTOR signallingresistancerapamycin (mTOR)human skeletal muscleprotein synthesisimmunofluorescencemTOR cellular distributionprotein-protein co-localisationScience & TechnologyMultidisciplinary SciencesScience & Technology - Other TopicsAMINO-ACIDSCONTRACTIONS INCREASEMAMMALIAN TARGETGENE-PRODUCTSENHANCES MTORACTIVATIONPHOSPHORYLATIONINGESTIONTSC2TURNOVER
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