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Response surface optimisation of medium components for naringinase production from staphylococcus xylosus MAK 2

journal contribution
posted on 2010-09-01, 00:00 authored by Munish Puri, A Kaur, R Singh, A Singh
Response surface methodology was used to optimize the fermentation medium for enhancing naringinase production by Staphylococcus xylosus. The first step of this process involved the individual adjustment and optimization of various medium components at shake flask level. Sources of carbon (sucrose) and nitrogen (sodium nitrate), as well as an inducer (naringin) and pH levels were all found to be the important factors significantly affecting naringinase production. In the second step, a 22 full factorial central composite design was applied to determine the optimal levels of each of the significant variables. A second-order polynomial was derived by multiple regression analysis on the experimental data. Using this methodology, the optimum values for the critical components were obtained as follows: sucrose, 10.0%; sodium nitrate, 10.0%; pH 5.6; biomass concentration, 1.58%; and naringin, 0.50% (w/v), respectively. Under optimal conditions, the experimental naringinase production was 8.45 U/mL. The determination coefficients (R 2) were 0.9908 and 0.9950 for naringinase activity and biomass production, respectively, indicating an adequate degree of reliability in the model.

History

Journal

Applied biochemistry and biotechnology

Volume

162

Issue

1

Pagination

181 - 191

Publisher

Humana Press

Location

Totowa, United States

ISSN

0273-2289

eISSN

1559-0291

Language

eng

Notes

Published online: 8 September 2009

Publication classification

C1 Refereed article in a scholarly journal; C Journal article

Copyright notice

2009, Humana Press