Deakin University
Browse

File(s) not publicly available

Retention of glucocorticoid by isolated mammary tissue may complicate interpretation of results from in vitro experiments

journal contribution
posted on 1979-11-14, 00:00 authored by F F Bolander, Kevin Nicholas, Y J Topper
When mammary gland explants from mid-pregnant rats were incubated with insulin (5 μg/ml) and [3H]cortisol (5 μg/ml) for one day, the tissue accumulated 1.69 μg cortisol/g wet tissue. During a second incubation with insulin and prolactin (5 μg/ml), only 20% of the steroid was lost per day. Such retention of glucocorticoid had an important biological consequence: the tissue exposed for one day to insulin and cortisol showed a transient stimulation of casein synthesis during a subsequent, five-day incubation with insulin and prolactin. No casein synthesis was detected, if the first culture medium contained only insulin. In conclusion, mammary gland explants from mid-pregnant rats require a glucocorticoid for casein synthesis, but this requirement may be obscured if the explants are initially incubated in medium containing cortisol, since they are capable of accumulating and retaining this steroid. Similar interpretative difficulties may arise in studies on other steroid-tissue relationships. © 1979.

History

Journal

Biochemical and Biophysical Research Communications

Volume

91

Issue

1

Pagination

247 - 252

ISSN

0006-291X

eISSN

1090-2104

Usage metrics

    Research Publications

    Categories

    No categories selected

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC