elnathan-roleofactin-2022.pdf (1.78 MB)
Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes
journal contribution
posted on 2022-11-15, 03:40 authored by Y Chen, HZ Yoh, AR Shokouhi, T Murayama, K Suu, Y Morikawa, NH Voelcker, Roey ElnathanRoey ElnathanAbstractNanofabrication technologies have been recently applied to the development of engineered nano–bio interfaces for manipulating complex cellular processes. In particular, vertically configurated nanostructures such as nanoneedles (NNs) have been adopted for a variety of biological applications such as mechanotransduction, biosensing, and intracellular delivery. Despite their success in delivering a diverse range of biomolecules into cells, the mechanisms for NN-mediated cargo transport remain to be elucidated. Recent studies have suggested that cytoskeletal elements are involved in generating a tight and functional cell–NN interface that can influence cargo delivery. In this study, by inhibiting actin dynamics using two drugs—cytochalasin D (Cyto D) and jasplakinolide (Jas), we demonstrate that the actin cytoskeleton plays an important role in mRNA delivery mediated by silicon nanotubes (SiNTs). Specifically, actin inhibition 12 h before SiNT-cellular interfacing (pre-interface treatment) significantly dampens mRNA delivery (with efficiencies dropping to 17.2% for Cyto D and 33.1% for Jas) into mouse fibroblast GPE86 cells, compared to that of untreated controls (86.9%). However, actin inhibition initiated 2 h after the establishment of GPE86 cell–SiNT interface (post-interface treatment), has negligible impact on mRNA transfection, maintaining > 80% efficiency for both Cyto D and Jas treatment groups. The results contribute to understanding potential mechanisms involved in NN-mediated intracellular delivery, providing insights into strategic design of cell–nano interfacing under temporal control for improved effectiveness.
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Journal
Journal of NanobiotechnologyVolume
20Article number
406Pagination
1-11Location
Bingley, Eng.Publisher DOI
ISSN
1477-3155eISSN
1477-3155Language
EnglishPublication classification
C1 Refereed article in a scholarly journalIssue
1Publisher
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Actin inhibitionBiotechnology & Applied MicrobiologyCURVATURECytochalasin DCytoskeletonEFFICIENCYELECTROPORATIONENDOCYTOSISIntracellular deliveryINTRACELLULAR DELIVERYJasplakinolideLife Sciences & BiomedicineLIVING CELLSmRNANanoinjectionNANONEEDLESNanoscience & NanotechnologyNANOWIRE ARRAYSPLASMA-MEMBRANEScience & TechnologyScience & Technology - Other TopicsSilicon nanotubesTRANSFECTIONActin CytoskeletonActinsAnimalsMechanotransduction, CellularMiceNanotubesRNA, MessengerSiliconBioengineeringNanotechnology1.1 Normal biological development and functioningGeneric health relevance110103 Medical Biochemistry: Inorganic Elements and CompoundsTechnology
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