File(s) under permanent embargo
Role of alveolar type II cells and of surfactant-associated protein C mRNA levels in the pathogenesis of respiratory distress in mink kits infected with Aleutian mink disease parvovirus
journal contribution
posted on 1994-04-01, 00:00 authored by B Viuff, B Aasted, Soren AlexandersenSoren AlexandersenNeonatal mink kits infected with Aleutian mink disease parvovirus (ADV) develop an acute interstitial pneumonia with clinical symptoms and pathological lesions that resemble those seen in preterm human infants with respiratory distress syndrome and in human adults with adult respiratory distress syndrome. We have previously suggested that ADV replicates in the alveolar type II epithelial cells of the lung. By using double in situ hybridization, with the simultaneous use of a probe to detect ADV replication and a probe to demonstrate alveolar type II cells, we now confirm this hypothesis. Furthermore, Northern (RNA) blot hybridization showed that the infection caused a significant decrease of surfactant-associated protein C mRNA produced by the alveolar type II cells. We therefore suggest that the severe clinical symptoms and pathological changes characterized by hyaline membrane formation observed in ADV-infected mink kits are caused by a dysfunction of alveolar surfactant similar to that observed in respiratory distress syndrome in preterm infants. However, in the infected mink kits the dysfunction is due to the replication of ADV in the lungs, whereas the dysfunction of surfactant in preterm infants is due to lung immaturity.
History
Journal
Journal of VirologyVolume
68Issue
4Pagination
2720 - 2725Publisher
American Society for MicrobiologyLocation
Washington, D.C.ISSN
0022-538XLanguage
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1994, American Society for MicrobiologyUsage metrics
Categories
No categories selectedKeywords
Aleutian Mink DiseaseAleutian Mink Disease VirusAnimalsAnimals, NewbornDNA, ViralHumansIn Situ HybridizationInfant, NewbornMinkMolecular Sequence DataProteolipidsPulmonary AlveoliPulmonary SurfactantsRNA ProbesRNA, MessengerRespiratory Distress Syndrome, NewbornScience & TechnologyLife Sciences & BiomedicineVirologyACUTE INTERSTITIAL PNEUMONIAPULMONARY SURFACTANTHUMAN-LUNGINSITU HYBRIDIZATIONVIRAL REPLICATIONMESSENGER-RNALOCALIZATIONVIRUSPROLIFERATIONSEQUENCE
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC