Information about the ecological functional roles of native predators may help inform the conservation of wildlife and pest management. If predators show preferences for certain prey, such as invasive species, this could potentially be used as a conservation tool to help restore degraded (e.g. overgrazed) ecosystems via the reintroduction of native predators and suppression of exotic prey (e.g. introduced herbivores). The diet of spotted-tailed quolls was studied in a fenced reserve in south-eastern Australia where native mammals have been reintroduced, foxes and cats removed, but invasive European rabbits still persist. A total of 80 scats were collected over 12 months and analysis of macroscopic prey remains was conducted to determine diet. Rabbits were by far the most commonly consumed prey species by volume (~76%) and frequency (~60%), followed by brushtail possums (~11% for both volume and frequency), and other small and medium-sized native mammals in much smaller amounts. Quoll scat analysis revealed 10 mammal species in total, eight of which were native. Bird, reptile and invertebrate remains were uncommon in quoll scats. This suggests that spotted-tailed quolls may show a preference for preying on invasive European rabbits in certain contexts, and this could potentially be used as part of quoll reintroductions to aid rabbit population suppression and ecosystem restoration.