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SARS-CoV-2 genomic and subgenomic RNAs in diagnostic samples are not an indicator of active replication

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Version 2 2024-06-05, 23:52
Version 1 2020-12-06, 17:06
journal contribution
posted on 2024-06-05, 23:52 authored by Soren AlexandersenSoren Alexandersen, Anthony Chamings, Tarka Raj Bhatta
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first detected in late December 2019 and has spread worldwide. Coronaviruses are enveloped, positive sense, single-stranded RNA viruses and employ a complicated pattern of virus genome length RNA replication as well as transcription of genome length and leader containing subgenomic RNAs. Although not fully understood, both replication and transcription are thought to take place in so-called double-membrane vesicles in the cytoplasm of infected cells. Here we show detection of SARS-CoV-2 subgenomic RNAs in diagnostic samples up to 17 days after initial detection of infection and provide evidence for their nuclease resistance and protection by cellular membranes suggesting that detection of subgenomic RNAs in such samples may not be a suitable indicator of active coronavirus replication/infection.

History

Journal

Nature Communications

Volume

11

Article number

6059

Pagination

1-13

Location

London, Eng.

Open access

  • Yes

eISSN

2041-1723

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Publisher

Nature Publishing Group

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