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Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection
journal contribution
posted on 2015-06-01, 00:00 authored by J Pandohee, Brendan HollandBrendan Holland, Bingshan Li, T Tsuzuki, Paul Stevenson, Neil BarnettNeil Barnett, J R Pearson, O A Jones, Xavier ConlanXavier ConlanWidely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.
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Journal
Journal of separation scienceVolume
38Issue
12Pagination
2024 - 2032Publisher
WileyLocation
Berlin, GermanyPublisher DOI
eISSN
1615-9314Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, WileyUsage metrics
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