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Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins

Version 2 2024-06-03, 21:28
Version 1 2017-05-16, 15:53
journal contribution
posted on 2024-06-03, 21:28 authored by IG Macreadie, LA Castelli, Alister WardAlister Ward, MJR Stark, AA Azad
A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of β-lactamase and determine optimal conditions for its secretion into the culture media.

History

Journal

Biotechnology letters

Volume

15

Pagination

213-218

Location

Dordrecht, The Netherlands

ISSN

0141-5492

eISSN

1573-6776

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1993, Science and Technology Letters

Issue

3

Publisher

Kluwer Academic Publishers

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