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Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins
journal contribution
posted on 1993-03-01, 00:00 authored by I G Macreadie, L A Castelli, Alister WardAlister Ward, M J R Stark, A A AzadA convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of β-lactamase and determine optimal conditions for its secretion into the culture media.