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Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection

journal contribution
posted on 2003-03-17, 00:00 authored by Jason Wesley Costin, Paul FrancisPaul Francis, S Lewis
The determination of the amino acids proline, histidine, tyrosine, arginine, phenylalanine and tryptophan using flow injection analysis (FIA) with chemiluminescence detection is described. Proline was the only amino acid to exhibit chemiluminescence with the tris(2,2-bipyridyl)ruthenium(III) reaction at pH 10. While, histidine was found to selectively enhance the reaction of luminol with Mn(II) salts in a basic medium. Acidic potassium permanganate chemiluminescence was able to selectively determine tyrosine at pH 6.75. Low pressure separations using a C18 guard column allowed the simultaneous determination of tyrosine and tryptophan or phenylalanine and tryptophan with acidic potassium permanganate and copper(II)–amino acid–hydrogen peroxide chemiluminescence, respectively. Precision for each method was less than 3.9% (R.S.D.) for five replicates of a standard (1×10−5 M) and the detection limits ranged between 4×10−9 and 7×10−6 M. Preliminary investigations revealed that the methodology developed was able to selectively determine the individual amino acids in an equimolar mixture of the 20 naturally occurring amino acids.

History

Journal

Analytica chimica acta

Volume

480

Issue

1

Pagination

67 - 77

Publisher

Elsevier BV

Location

Amsterdam, The Netherlands

ISSN

0003-2670

eISSN

1873-4324

Language

eng

Publication classification

C1 Refereed article in a scholarly journal