rivera-seleniumenriched-2014.pdf (474.26 kB)
Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats
journal contribution
posted on 2014-06-24, 00:00 authored by T Maseko, F R Dunshea, K Howell, H-J Cho, Leni RiveraLeni Rivera, J B Furness, K NgDietary effects of organic Se supplementation in the form of Se-enriched Agaricus bisporus mushroom on ileal mucosal permeability and antioxidant selenoenzymes status in heat induced oxidative stress in rats were evaluated. Acute heat stress (40 °C, 21% relative humidity, 90 min exposure) increased ileum baseline short circuit current (Isc; 2.40-fold) and epithelial conductance (Ge; 2.74-fold). Dietary supplementation with Se-enriched A. bisporus (1 µg Se/g feed) reduced (p < 0.05) ileum Isc and Ge during heat stress to 1.74 and 1.91 fold, respectively, indicating protection from heat stress-induced mucosal permeability increase. The expression of ileum glutathione peroxidase (GPx-) 1 and 2 mRNAs were up-regulated (p < 0.05) by 1.90 and 1.87-fold, respectively, for non-heat stress rats on the Se-enriched diet relative to the control. The interplay between heat stress and dietary Se is complex. For rats on the control diet, heat stress alone increased ileum expression of GPx-1 (2.33-fold) and GPx-2 (2.23-fold) relative to thermoneutral conditions. For rats on the Se-enriched diet, heat stress increased (p < 0.05) GPx-1 expression only. Rats on Se-enriched + α-tocopherol diet exhibited increased expression of both genes (p < 0.05). Thus, dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.
History
Journal
NutrientsVolume
6Issue
6Pagination
2478 - 2492Publisher
MDPILocation
Basel, SwitzerlandPublisher DOI
eISSN
2072-6643Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2014, The AuthorsUsage metrics
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AgaricusAnimal FeedAnimalsGene Expression Regulation, EnzymologicGlutathione PeroxidaseHot TemperatureIntestinesMaleOxidative StressPermeabilityRandom AllocationRatsRats, Sprague-DawleyReal-Time Polymerase Chain ReactionSeleniumUp-RegulationSe-enriched mushroomAgaricus bisporusheat stressorganic Segut permeabilityglutathione peroxidise-1glutathione peroxidise-2α-tocopherolScience & TechnologyLife Sciences & BiomedicineNutrition & Dieteticsalpha-tocopherolEXPRESSIONCOLONINTESTINEDISEASECANCERLIVER
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