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Self-organised nanoarchitecture of titanium surfaces influences the attachment of Staphylococcus aureus and Pseudomonas aeruginosa bacteria
journal contribution
posted on 2015-08-01, 00:00 authored by V K Truong, V T Pham, Alexander Medvedev, Rimma LapovokRimma Lapovok, Y Estrin, T C Lowe, V Baulin, V Boshkovikj, C J Fluke, R J Crawford, E P IvanovaThe surface nanotopography and architecture of medical implant devices are important factors that can control the extent of bacterial attachment. The ability to prevent bacterial attachment substantially reduces the possibility of a patient receiving an implant contracting an implant-borne infection. We now demonstrated that two bacterial strains, Staphylococcus aureus and Pseudomonas aeruginosa, exhibited different attachment affinities towards two types of molecularly smooth titanium surfaces each possessing a different nanoarchitecture. It was found that the attachment of S. aureus cells was not restricted on surfaces that had an average roughness (S a) less than 0.5 nm. In contrast, P. aeruginosa cells were found to be unable to colonise surfaces possessing an average roughness below 1 nm, unless sharp nanoprotrusions of approximately 20 nm in size and spaced 35.0 nm apart were present. It is postulated that the enhanced attachment of P. aeruginosa onto the surfaces possessing these nanoprotrusions was facilitated by the ability of the cell membrane to stretch over the tips of the nanoprotrusions as confirmed through computer simulation, together with a concomitant increase in the level of extracellular polymeric substance (EPS) being produced by the bacterial cells.
History
Journal
Applied microbiology and biotechnologyVolume
99Issue
16Pagination
6831 - 6840Publisher
SpringerLocation
New York, N.Y.Publisher DOI
ISSN
1432-0614Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2015, SpringerUsage metrics
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No categories selectedKeywords
Bacterial attachmentMolecularly smooth surfacesPseudomonas aeruginosaStaphylococcus aureusSurface nanoarchitectureScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyATOMIC-FORCE MICROSCOPYBIOFILM FORMATIONSTAINLESS-STEELNANOSCALE ROUGHNESSTOPOGRAPHYCELLSQUANTIFICATIONADHESIONOSTEOBLASTRETENTION
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