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Sequence analysis of a salt tolerant metagenomic clone

journal contribution
posted on 2010-06-01, 00:00 authored by R Kapardar, R Ranjan, Munish Puri, R Sharma

Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from proteobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-dependent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.

History

Journal

Indian journal of microbiology

Volume

50

Issue

2

Pagination

212 - 215

Publisher

Springer (India) Private Ltd

Location

New Delhi, India

ISSN

0046-8991

eISSN

0973-7715

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2010, Association of Microbiologists of India