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Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system

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journal contribution
posted on 2024-06-18, 21:24 authored by Taehee Kang, Chulhun Park, Nileshkumar Meghani, Thao TD Tran, Phuong HL Tran, Beom-Jin Lee
Cellular properties and microenvironments, as well as the characteristics of nanoparticles (NPs), affect the cellular uptake and cytotoxic effects of drug-loaded NPs. Since there is fluid flow in the human blood system, fluid flow also affects the drug delivery efficiency of NPs. This study aimed to evaluate the cellular behaviors of drug-loaded soft NPs on A549 cancer cells under different levels of shear stress (0.5, 5, and 50 dynes/cm2) in the biomimetic microfluidic system. The soft self-assembled NPs were formed by the gelatin–oleic conjugate (GOC). The poorly water-soluble coumarin-6 or paclitaxel (PTX) were used as model markers for encapsulation within self-assembled NPs (C-GONs or PTX-GONs, respectively). The cellular uptake of C-GONs was found to be improved with shear-stress dependence. The inhibitory concentration (IC50) of PTX-GONs at 0.5, 5, and 50 dynes/cm2 was 0.106 µg/mL, 0.108 µg/mL, and 0.091 µg/mL, respectively, as compared to 0.138 µg/mL in a static condition. The cell killing efficiency of PTX-GONs was increased in the highest shear stress of 50 dynes/cm2 in the static condition, and other levels of shear stress in dynamic conditions.

History

Journal

Pharmaceutics

Volume

12

Article number

555

Location

Basel, Switzerland

Open access

  • Yes

ISSN

1999-4923

eISSN

1999-4923

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2020, The Authors

Issue

6

Publisher

MDPI AG