Deakin University
Browse

File(s) not publicly available

Short communication: bovine-derived proteins activate STAT3 in human skeletal muscle in vitro

Version 2 2024-06-05, 10:51
Version 1 2015-07-23, 16:01
journal contribution
posted on 2024-06-05, 10:51 authored by M Caldow, MR Digby, D Cameron-Smith
Bovine milk contains biologically active peptides that may modulate growth and development within humans. In this study, targeted bovine-derived proteins were evaluated for their effects on signal transducer and activator of transcription-3 (STAT3) phosphorylation in human skeletal muscle cells. Following an acute exposure, bovine-derived acidic fibroblast growth factor-1 (FGF) and leukemia inhibitory factor (LIF) activated STAT3 in differentiating myotubes. Chronic exposure to FGF and LIF during the proliferative phase reduced myoblast proliferation and elevated MyoD and creatine kinase (CKM) mRNA expression without altering apoptotic genes. In mature myotubes, neither FGF nor LIF elicited any action. Together, these data indicate that a reduction in proliferation in the presence of bovine-derived FGF or LIF may stimulate early maturation of myoblasts.

History

Journal

Journal of dairy science

Volume

98

Pagination

3016-3019

Location

Amsterdam, The Netherlands

ISSN

0022-0302

eISSN

1525-3198

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2015, American Dairy Science Association

Issue

5

Publisher

Elsevier