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Signaling responses after varying sequencing of strength and endurance training in a fed state
journal contribution
posted on 2016-10-01, 00:00 authored by Thomas W Jones, Ian H Walshe, Lee HamiltonLee Hamilton, Glyn Howatson, Mark Russell, Oliver J Price, Alan St Clair Gibson, Duncan N FrenchPURPOSE: To compare anabolic signaling responses to differing sequences of concurrent strength and endurance training in a fed state. METHODS: Eighteen resistance-trained men were randomly assigned to the following experimental conditions: strength training (ST), strength followed by endurance training (ST-END), or endurance followed by strength training (END-ST). Muscle tissue samples were taken from the vastus lateralis before each exercise protocol, on cessation of exercise, and 1 h after cessation of strength training. Tissue was analyzed for total and phosphorylated (p-) signaling proteins linked to the mTOR and AMPK networks. RESULTS: Strength-training performance was similar between ST, ST-END, and END-ST. p-S6k1 was elevated from baseline 1 h posttraining in ST and ST-END (both P < .05). p-4E-BP1 was significantly lower than baseline post-ST (P = .01), whereas at 1 h postexercise in the ST-END condition p-4E-BP1 was significantly greater than postexercise (P = .04). p-ACC was elevated from baseline both postexercise and 1 h postexercise (both P < .05) in the END-ST condition. AMPK, mTOR, p38, PKB, and eEF2 responded similarly to ST, ST-END, and END-ST. Signaling responses to ST, ST-END, and END were largely similar. As such it cannot be ascertained which sequence of concurrent strength and endurance training is most favorable in promoting anabolic signaling. CONCLUSIONS: In the case of the current study an acute bout of concurrent training of differing sequences elicited similar responses of the AMPK and mTOR networks.
History
Journal
International journal of sports physiology and performanceVolume
11Issue
7Pagination
868 - 875Publisher
Human KineticsLocation
Champaign, Ill.Publisher DOI
ISSN
1555-0265eISSN
1555-0273Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2016, Human Kinetics, Inc.Usage metrics
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combined exercisemTORAMPKexercise orderanabolic signalingAMP-Activated Protein KinasesEatingMuscle StrengthMuscle, SkeletalPhysical EnduranceResistance TrainingSignal TransductionTOR Serine-Threonine KinasesScience & TechnologyLife Sciences & BiomedicinePhysiologySport SciencesHUMAN SKELETAL-MUSCLERESISTANCE EXERCISEPROTEIN-SYNTHESISMOLECULAR RESPONSESGLYCOGEN AVAILABILITYADAPTIVE RESPONSESAEROBIC EXERCISEDOSE-RESPONSEPHOSPHORYLATIONACTIVATION
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