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Simple and cost-effective determination of ciprofloxacin hydrochloride by electrical micro-titration

journal contribution
posted on 2017-07-01, 00:00 authored by X Z Zhang, M S Huang, Q Q Yang, D S Ding, J Zhao, Wenrong YangWenrong Yang, K M Qu
By employing an electrical micro-titration system, in which a capacitively coupled contactless conductivity detector (C 4 D) was used to monitor the reaction process in real time, herein a novel method for determining ciprofloxacin hydrochloride (CIPHCl) was developed for the first time. Mode 1: Standard CIPHCl solutions at different concentrations were loaded into reaction cells, respectively, and were titrated with standard Ag + . Upon the titration, the formation of a precipitate alters the number of ions in the solution, raising the change of conductivity, which was monitored by a special C 4 D to construct a titration curve. The endpoint of the titration was located from the peak of the curve. Between the elapsed time and the initial concentration of titrand, a linear relationship was established over the range of 2.0-8.0mmol/L. Mode 2: Standard Fe 3+ took the place of Ag + , and was used as titrant to recognize ciprofloxacin contributed to the formation of complexation, which also resulting a change of solution conductivity. Under optimized conditions, a working range of 1.0-5.0mmol/L CIPHCl was found. Because the reaction solutions were isolated from the working electrodes, this pioneer work shows significant simplicity and cost-effectiveness, by eliminating the requirements for detector exchange/renewal between different measurements, and by involving no auxiliary chemicals. Both of the two approaches were applied successfully to determine CIPHCl in tablet samples. And the results were in good agreement with those obtained by reference method.



Chinese chemical letters






1406 - 1412




Amsterdam, The Netherlands





Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2017, Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences