File(s) under permanent embargo
Simple, quantitative method for low molecular weight dissolved organic matter extracted from natural waters based upon high performance counter-current chromatography
journal contribution
posted on 2016-02-25, 00:00 authored by A Rojas, S Sandron, R Wilson, N W Davies, P R Haddad, Robert ShellieRobert Shellie, P N Nesterenko, B Paull© 2016 Elsevier B.V. A simple, high-performance counter-current chromatography method with sequential UV absorbance (254 nm) and evaporative light scattering detection (ELSD) was developed for the quantification of pre-extracted low molecular weight dissolved organic matter (DOM) extracted from natural waters. The method requires solid-phase extraction (SPE) extraction of only small volumes of water samples, here using poly(styrenedivinylbenzene)-based extraction cartridges (Varian PPL). The extracted and concentrated DOM was quantified using reversed-phase high-performance counter-current chromatography (HPCCC), with a water/methanol (5:5) mobile phase and hexane/ethyl acetate (3:7) stationary phase. The critical chromatographic parameters were optimised, applying a revolution speed of 1900 rpm and a flow-rate of 1 mL min-1. Under these conditions, 50 μL of extracted DOM solution could be injected and quantified using calibration against a reference natural dissolved material (Suwannee River), based upon UV absorbance at 254 nm and ELSD detection. Both detection methods provided excellent linearity (R2> 0.995) for DOM across the concentration ranges of interest, with limits of detection of 4 μg ml-1and 7 μg ml-1for ELSD and UV absorbance, respectively. The method was validated for peak area precision (<5%), and accuracy and recovery based upon spiking seawater samples prior to extraction, together with DOM solutions post-extraction (>95% recovery). The developed method was applied to the determination of the concentration of DOM in seawater, based upon initial sample volumes as small as 20 mL.
History
Journal
Analytica Chimica ActaVolume
909Pagination
129 - 138Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0003-2670eISSN
1873-4324Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2016, ElsevierUsage metrics
Categories
No categories selectedKeywords
High-performance counter-current chromatographyDissolved organic matterSuwannee river natural organic matterQuantificationEvaporative light scattering detectionScience & TechnologyPhysical SciencesChemistry, AnalyticalChemistrySIZE-EXCLUSION CHROMATOGRAPHYLIQUID-CHROMATOGRAPHYMASS-SPECTROMETRYFRACTIONATIONCARBONOCEANSPECTROSCOPYSEPARATIONNITROGEN
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC