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Simultaneous conjugal transfer in Lactococcus to genes involved in bacteriocin production and reduced susceptibility to bacteriophages

journal contribution
posted on 2024-05-02, 01:09 authored by IB Powell, Alister WardAlister Ward, AJ Hillier, BE Davidson
Conjugal matings were performed between Lactococcus lactis DRC1 (a lactose-fermenting (Lac+), bacteriocin-producing (Bac+) strain) and L. lactis HID113 (Lac- and Bac-). Transconjugant derivatives of HID113 were identified on the basis of lactose fermentation, resistance to the DRC1 bacteriocin (dricin) or reduced sensitivity to phage sk1. Regardless of how they were identified, all transconjugants gave fewer and smaller plaques with phages c2 and sk1 than did HID113. All but one of 275 transconjugants tested also produced dricin, suggesting some functional relationship or close genetic linkage between the reduced phage sensitivity and dricin production and resistance. Some transconjugants were also Lac+, but this property was unstable.

History

Journal

Fems Microbiology Letters

Volume

60

Pagination

209-213

Location

Oxford, Eng.

ISSN

0378-1097

eISSN

1574-6968

Language

eng

Publication classification

CN.1 Other journal article

Copyright notice

1990, Oxford University Press

Issue

1-2

Publisher

Oxford University Press