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Sodium alginate/magnesium oxide nanocomposite scaffolds for bone tissue engineering
journal contribution
posted on 2018-09-01, 00:00 authored by Bijan Nasri-Nasrabadi, Akif KaynakAkif Kaynak, Pejman Heidarian, Z Komeily-Nia, M Mehrasa, H Salehi, Abbas KouzaniAbbas Kouzani© 2018 John Wiley & Sons, Ltd. A simple 2-step method, consisting of film casting and polyvinyl alcohol leaching, is proposed to prepare magnesium oxide (MO) nanoparticle-reinforced sodium alginate scaffolds with right properties for bone tissue engineering. The cytocompatibility of the as-prepared scaffolds was also evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide yellow tetrazole assay test, wherein chondrocyte cells had been considered as target cells. According to the results, the ensuing sodium alginate nanocomposites, containing 4-wt% MO nanoparticles, demonstrated the highest physical and mechanical properties after leaching step. The Young modulus of sodium alginate/4-wt% MO was improved about 44%, in comparison with that of the pure alginate sample. Furthermore, incorporating MO nanoparticles up to 4 wt% controlled the liquid uptake capacity of scaffolds vis-à-vis the resultant pure sodium alginate sample. Moreover, with increasing the nanoparticle content, the antibacterial properties of scaffolds enhanced, but their degradation rates under in vitro conditions tapered off. With the introduction of 3- and 4-wt% MO, the average diameter of the bacterial zone of the scaffold samples reduced to less than 10 mm2, suggesting an insensitive antimicrobial performance, compared with the pure sodium alginate and the samples with 1- and 2-wt% MO content, which exhibit antimicrobial sensitivity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide assay test also revealed the cultivated chondrocyte cells on the 4-wt% MO nanoparticle-reinforced scaffold possessed better interaction as well as appropriate cell attachment and proliferation than the pristine sodium alginate sample.
History
Journal
Polymers for advanced technologiesVolume
29Issue
9Pagination
2553 - 2559Publisher
WileyLocation
Chichester, Eng.Publisher DOI
ISSN
1042-7147eISSN
1099-1581Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, John Wiley & SonsUsage metrics
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