File(s) under permanent embargo

Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity

journal contribution
posted on 01.07.2007, 00:00 authored by K Fujita, D MacFarlane, Maria ForsythMaria Forsyth, M Yoshizawa-Fujita, K Murata, N Nakamura, H Ohno
Hydrated ionic liquids (ILs) were prepared by adding appropriate amounts of water to hydrophilic ILs. Some hydrated ILs show excellent solubilizing ability for proteins, keeping the basic properties of ILs. The solubility of cytochrome c (cyt c) depended on the structure of the component ions. When component anions have oxo acid residues, the resulting hydrated ILs solubilize cyt c quite well. In such hydrated ILs, the structure and activity of cyt c is influenced by the kosmotropicity of the component ions. We synthesized ILs from various ions having different kosmotropicity, including dihydrogen phosphate (dhp), dibutylphosphate, acetate, lactate, and methanesulfonate as anions. The activity of the dissolved cyt c depends on the permutations of kosmotropicity of the component ions. cyt c shows no structural change and retains its activity when dissolved in the hydrated choline dhp, which is an excellent combination of chaotropic cation and kosmotropic anion. Furthermore, cyt c dissolved in the hydrated choline dhp remained in a native state and was active after 18 months of storage at room temperature.

History

Journal

Biomacromolecules

Volume

8

Issue

7

Pagination

2080 - 2086

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

1525-7797

eISSN

1526-4602

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2007, American Chemical Society