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Spatial distribution of nutritive and nonnutritive vascular routes in perfused rat hindlimb muscle using microspheres
journal contribution
posted on 2001-01-01, 00:00 authored by Michelle KeskeMichelle Keske, S Rattigan, M G Clark, S L Bernard, R W GlennySkeletal muscle appears to have two vascular flow routes, nutritive and nonnutritive, where the balance of flow is controlled by vasoconstrictors. In the present study, spatial distributions of the two flow routes in muscles of the perfused rat hindlimb were attempted using fluorescent microspheres (15 microm in diameter). Microspheres were injected during steady-state perfusion with norepinephrine (proposed recruiter of nutritive flow), serotonin (proposed recruiter of nonnutritive flow), or vehicle. The three-dimensional location of individual microspheres in representative muscles was determined using a Fluorescent Imaging CryoMicrotome. Norepinephrine and serotonin each increased perfusion pressure (P < 0.05) but stimulated and inhibited oxygen consumption (P < 0.05), respectively. The distribution of microspheres lodged in muscle was independent of the agent used. Spatial perfusion indices for norepinephrine, serotonin, and vehicle did not differ from each other. Similarly, there was no difference in these indices for a theoretical distribution where microspheres were deliberately positioned in muscle bundle capillaries or interfibrillar connective tissue vessels. We conclude that the nutritive and nonnutritive flow routes are distributed throughout muscle sections consistent with their locations in muscle bundle capillaries and interfibrillar connective tissue, respectively.
History
Journal
Microvascular researchVolume
61Issue
1Pagination
111 - 121Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0026-2862Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, Academic PressUsage metrics
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No categories selectedKeywords
AnimalsHindlimbMaleMicrocirculationMicroscopy, FluorescenceMicrospheresMuscle, SkeletalRatsnutritive flownonnutritive flowtwo circulatory systems in musclemicrosphere embolismfluorescent microspheresconnective tissue vesselsScience & TechnologyLife Sciences & BiomedicinePeripheral Vascular DiseaseCardiovascular System & CardiologyBLOOD-FLOW DISTRIBUTIONRABBIT TENUISSIMUS MUSCLESKELETAL-MUSCLEOXYGEN-UPTAKEMETABOLISMSEROTONINVASOCONSTRICTIONNOREPINEPHRINESTIMULATIONblood flow
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