wadley-stageof-2012.pdf (282.13 kB)
Stage of perinatal development regulates skeletal muscle mitochondrial biogenesis and myogenic regulatory factor genes with little impact of growth restriction or cross-fostering
journal contribution
posted on 2012-02-01, 00:00 authored by R Laker, Glenn WadleyGlenn Wadley, G McConell, M WlodekFoetal growth restriction impairs skeletal muscle development and adult muscle mitochondrial biogenesis. We hypothesized that key genes involved in muscle development and mitochondrial biogenesis would be altered following uteroplacental insufficiency in rat pups, and improving postnatal nutrition by cross-fostering would ameliorate these deficits. Bilateral uterine vessel ligation (Restricted) or sham (Control) surgery was performed on day 18 of gestation. Males and females were investigated at day 20 of gestation (E20), 1 (PN1), 7 (PN7) and 35 (PN35) days postnatally. A separate cohort of Control and Restricted pups were cross-fostered onto a different Control or Restricted mother and examined at PN7. In both sexes, peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1α (PGC-1α), cytochrome c oxidase subunits 3 and 4 (COX III and IV) and myogenic regulatory factor 4 expression increased from late gestation to postnatal life, whereas mitochondrial transcription factor A, myogenic differentiation 1 (MyoD), myogenin and insulin-like growth factor I (IGF-I) decreased. Foetal growth restriction increased MyoD mRNA in females at PN7, whereas in males IGF-I mRNA was higher at E20 and PN1. Cross-fostering Restricted pups onto a Control mother significantly increased COX III mRNA in males and COX IV mRNA in both sexes above controls with little effect on other genes. Developmental age appears to be a major factor regulating skeletal muscle mitochondrial and developmental genes, with growth restriction and cross-fostering having only subtle effects. It therefore appears that reductions in adult mitochondrial biogenesis markers likely develop after weaning.
History
Journal
Journal of development origins of health and diseaseVolume
3Issue
1Pagination
39 - 51Publisher
Cambridge University PressLocation
Cambridge, EnglandPublisher DOI
ISSN
2040-1744Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2011, Cambridge University Press and the International Society for Developmental Origins of Health and DiseaseUsage metrics
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gene expressiongrowthgrowth restrictionskeletal musclereducing litter sizebirth weightScience & TechnologyLife Sciences & BiomedicinePublic, Environmental & Occupational HealthUTEROPLACENTAL INSUFFICIENCYFETAL-GROWTHGLUCOSE-TOLERANCEPOSTNATAL-GROWTHPLACENTAL GROWTHBIRTH-WEIGHTMATERNAL UNDERNUTRITIONPRENATAL UNDERNUTRITIONINSULIN-RESISTANCE
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