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Star anise (Illicium verum Hook. f.) as quorum sensing and biofilm formation inhibitor on foodborne bacteria: study in milk

Version 2 2024-06-05, 09:28
Version 1 2017-05-16, 15:18
journal contribution
posted on 2024-06-05, 09:28 authored by MRT Rahman, Z Lou, J Zhang, F Yu, YP Timilsena, C Zhang, Y Zhang, AM Bakry
Bacteria use quorum sensing (QS) systems to communicate with each other and regulate microbial group behavior, such as the secretion of virulence factors, including biofilm formation. In order to explore safe, edible agents, the potential of star anise (SA) as an anti-QS and antibiofilm agent and its possible application in milk safety were investigated. Staphylococcus aureus , Salmonella Typhimurium, Pseudomonas aeruginosa , and biosensor strain Chromobacterium violaceum were selected as test strains for QS, biofilm, and exopolysaccharide assays. The percent acidities and total plate counts were determined to evaluate the quality of biofilm-inoculated and noninoculated milk. The yield of SA extraction was 25.90% ± 0.2% (w/w). At sub-MIC, SA extract did not show any effect on bacterial growth. The production of violacein was inhibited by 89% by SA extract. The extract also inhibited the formation of biofilm by up to 87% in a dose-dependent manner. Inhibition rates of 70.45%, 42.82%, and 35.66% were found for exopolysaccharide production. The swarming motility of S. aureus was reduced by about 95.9% by SA extract. Confocal laser scanning microscopy analysis confirmed that the development of biofilm architecture was hampered. It was found that SA extract could delay the spoilage of milk. In the endeavor to avoid drug resistance, pathogenesis, and resistance to biocides while improving food safety and avoiding health hazard issues arising from synthetic chemicals, SA extract could be used as a potential QS and biofilm inhibitor.

History

Journal

Journal of food protection

Volume

80

Pagination

645-653

Location

Ames, Iowa

ISSN

0362-028X

eISSN

1944-9097

Language

eng

Publication classification

C Journal article, C1.1 Refereed article in a scholarly journal

Copyright notice

2017, International Association for Food Protection

Issue

4

Publisher

International Association of Milk, Food, and Environmental Sanitarians