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Stepwise synthesis of Gly−Gly−His on gold surfaces modified with mixed self-assembled monolayers

journal contribution
posted on 2005-01-04, 00:00 authored by Wenrong YangWenrong Yang, D Hibbert, R Zhang, G Willett, J Gooding
Peptide-modified electrode surfaces have been shown to have excellent recognition properties for metal ions. An efficient method of screening a potential peptide for its selectivity for a given metal would involve the synthesis of the peptide directly on the electrode surface. This paper outlines a procedure in which the tripeptide Gly−Gly−His was synthesized one amino acid at a time on a gold surface modified with a self-assembled monolayer of the mixed alkanethiolates 3-mercaptopropionic acid (MPA) and 3-mercaptopropane (MP). Electrochemistry and high-resolution mass spectrometry were used to elucidate the structure of the adsorbed species and follow the synthesis. The amino acids can be attached only to MPA, but the presence of a diluting unreactive molecule of MP reduces steric crowding about the reaction center. The maximum coverage of synthesized tripeptide occurs at a ratio of MPA/MP of 1:1.

History

Journal

Langmuir

Volume

21

Issue

1

Pagination

260 - 265

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

0743-7463

eISSN

1520-5827

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2005, American Chemical Society