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Stepwise synthesis of Gly−Gly−His on gold surfaces modified with mixed self-assembled monolayers
journal contribution
posted on 2005-01-04, 00:00 authored by Wenrong YangWenrong Yang, D Hibbert, R Zhang, G Willett, J GoodingPeptide-modified electrode surfaces have been shown to have excellent recognition properties for metal ions. An efficient method of screening a potential peptide for its selectivity for a given metal would involve the synthesis of the peptide directly on the electrode surface. This paper outlines a procedure in which the tripeptide Gly−Gly−His was synthesized one amino acid at a time on a gold surface modified with a self-assembled monolayer of the mixed alkanethiolates 3-mercaptopropionic acid (MPA) and 3-mercaptopropane (MP). Electrochemistry and high-resolution mass spectrometry were used to elucidate the structure of the adsorbed species and follow the synthesis. The amino acids can be attached only to MPA, but the presence of a diluting unreactive molecule of MP reduces steric crowding about the reaction center. The maximum coverage of synthesized tripeptide occurs at a ratio of MPA/MP of 1:1.
History
Journal
LangmuirVolume
21Issue
1Pagination
260 - 265Publisher
American Chemical SocietyLocation
Washington, D.C.Publisher DOI
ISSN
0743-7463eISSN
1520-5827Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2005, American Chemical SocietyUsage metrics
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