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Stimulation of cytokine production in human peripheral blood mononuclear cells by an aqueous Chlorella extract

journal contribution
posted on 2007-01-01, 00:00 authored by H Ewart, O Bloch, G Girouard, J Kralovec, Colin BarrowColin Barrow, G Ben-Yehudah, E Suarez, M Rapoport
CPE is an aqueous extract of the edible micro alga Chlorella pyrenoidosa, which has been shown to have immunostimulatory effects in vivo. In the present study, CPE was evaluated for an ability to stimulate cytokine production by human peripheral blood mononuclear cells (PBMC). PBMC from healthy individuals were treated ex vivo for 24 hours with 1, 10 and 100 μg/mL CPE. This resulted in a marked increase in the level of IL-10, a regulatory cytokine, and strong stimulation of the T-helper-1 (Th1) cell cytokines, IFN-γ and TNF-α. In contrast, stimulation of representative T-helper-2 (Th2) cell cytokines, IL-4 and IL-13, was minor. CPE (1, 10 or 100 μg/mL) did not cause a proliferation of human PBMC suggesting that enhanced secretion of cytokines was not secondary to an increase in cell number. We conclude that CPE stimulation of human PBMC induces a Th1-patterned cytokine response and a strong anti-inflammatory regulatory cytokine response, observations that await confirmation in vivo.

History

Journal

Planta med

Volume

73

Issue

8

Pagination

762 - 768

Publisher

Georg Thieme Verlag

Location

Stuttgart, Germany

ISSN

0032-0943

eISSN

1439-0221

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2007, Georg Thieme Verlag KG