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Structural-rheological characteristics of Chaplin E peptide at the air/water interface; a comparison with β-lactoglobulin and β-casein
journal contribution
posted on 2020-02-01, 00:00 authored by M Dokouhaki, Emma PrimeEmma Prime, G G Qiao, S Kasapis, L Day, S L Gras© 2019 Elsevier B.V. The Chaplin E peptide is a surface-active agent that can adsorb to the air/water interface and form interfacial films that display distinct interfacial properties as a function of pH. The ~2 nm thick homogeneous Chaplin E film formed under acidic conditions contains ordered structures that give a high dilatational elasticity. In contrast, the heterogeneous film formed under basic conditions contained fibrils resulting in a rough ~17 nm thick film with predominantly viscoelastic properties, probably due to the reduced intermolecular interactions. These fibrils were also susceptible to breakage, fragmenting into shorter fibrils, which gave a greater elasticity. The fibrils also lead to a greater shear viscosity compared to the ordered structures aligned within the Chaplin E film at pH 3.0. A higher stability was observed for the foam formed by the Chaplin E compared to the foam formed by β-lactoglobulin, consistent with the greater rheological properties observed for the Chaplin E film at the interface. Our findings suggest that Chaplin E has potential to provide long time stability to dispersions in food, consumer goods or pharmaceutical applications, forming films with greater rheological properties and at least similar thickness to those formed by other surface-active proteins such as β-casein and β-lactoglobulin.
History
Journal
International Journal of Biological MacromoleculesVolume
144Pagination
742 - 750Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0141-8130eISSN
1879-0003Language
engPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2020, ElsevierUsage metrics
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No categories selectedKeywords
Atomic force microscopyDilatational rheologyCanal viscometryChaplin ETransmission electron microscopyFoam stabilityScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemistry & Molecular BiologyChemistry, AppliedPolymer ScienceChemistryADSORBED PROTEIN LAYERSAIR-WATER-INTERFACESTREPTOMYCES-COELICOLORSHEAR RHEOLOGYPHFILMSFOAMHYDROPHOBINSTABILITYMONOGLYCERIDE
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