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Structural characterisation and bioactivities of hybrid carrageenan-like sulphated galactan from red alga Furcellaria lumbricalis
journal contribution
posted on 2011-01-01, 00:00 authored by B Yang, G Yu, X Zhao, W Ren, G Jiao, L Fang, Y Wang, G Du, C Tiller, G Girouard, Colin BarrowColin Barrow, H Ewart, J ZhangThe red alga, Furcellaria lumbricalis from the coast of the Prince Edward Island (PEI) in Atlantic Canada, was extracted with hot water and fractionated with 0.125. M KCl to obtain a carrageenan-like polysaccharide. The polysaccharide was further purified on ion-exchange and gel-permeation chromatography to yield a fraction (FB1) of uniform size and charge, with an average molecular weight of 428. kD. Oligosaccharides generated with acid hydrolysis of FB1 were sequenced using the electrospray ionisation collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS) technique. On the basis of chemical and spectroscopic analysis, FB1 was characterised to be composed of 1,4-linked 3,6-anhydro-galactose (40%), 1,3-linked 4-sulphated-galactose (30%), 1,3-linked galactose (20%), 1,4-linked galactose (8%) and 1,4-linked 3-O-methyl-galactose (2%), which makes it be a novel sulphated galactan hybrid. The β-secretase (BACE) inhibition and immunomodulation activities of FB1-derived oligosaccharides were evaluated in vitro.
History
Journal
Food chemistryVolume
124Issue
1Pagination
50 - 57Publisher
Elsevier BVLocation
Amsterdam, The NetherlandsISSN
0308-8146eISSN
1873-7072Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2010, ElsevierUsage metrics
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No categories selectedKeywords
bioactivitiesdepolymerisationFurcellaria lumbricalispurificationsulphated galactanScience & TechnologyPhysical SciencesLife Sciences & BiomedicineChemistry, AppliedFood Science & TechnologyNutrition & DieteticsChemistryAMYLOID PRECURSOR PROTEINH-1-NMR SPECTROSCOPYBETA-SECRETASEPOLYSACCHARIDESOLIGOSACCHARIDESRHODOPHYTAACID
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