Deakin University
Browse

File(s) under permanent embargo

Structural characterisation and bioactivities of hybrid carrageenan-like sulphated galactan from red alga Furcellaria lumbricalis

journal contribution
posted on 2011-01-01, 00:00 authored by B Yang, G Yu, X Zhao, W Ren, G Jiao, L Fang, Y Wang, G Du, C Tiller, G Girouard, Colin BarrowColin Barrow, H Ewart, J Zhang
The red alga, Furcellaria lumbricalis from the coast of the Prince Edward Island (PEI) in Atlantic Canada, was extracted with hot water and fractionated with 0.125. M KCl to obtain a carrageenan-like polysaccharide. The polysaccharide was further purified on ion-exchange and gel-permeation chromatography to yield a fraction (FB1) of uniform size and charge, with an average molecular weight of 428. kD. Oligosaccharides generated with acid hydrolysis of FB1 were sequenced using the electrospray ionisation collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS) technique. On the basis of chemical and spectroscopic analysis, FB1 was characterised to be composed of 1,4-linked 3,6-anhydro-galactose (40%), 1,3-linked 4-sulphated-galactose (30%), 1,3-linked galactose (20%), 1,4-linked galactose (8%) and 1,4-linked 3-O-methyl-galactose (2%), which makes it be a novel sulphated galactan hybrid. The β-secretase (BACE) inhibition and immunomodulation activities of FB1-derived oligosaccharides were evaluated in vitro.

History

Journal

Food chemistry

Volume

124

Issue

1

Pagination

50 - 57

Publisher

Elsevier BV

Location

Amsterdam, The Netherlands

ISSN

0308-8146

eISSN

1873-7072

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, Elsevier