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Structure and biodegradation mechanism of milled Bombyx mori silk particles

journal contribution
posted on 01.01.2012, 00:00 authored by Rangam RajkhowaRangam Rajkhowa, X Hu, Takuya Tsuzuki, D Kaplan, Xungai WangXungai Wang
The aim of this study was to understand the structure and biodegradation relationships of silk particles intended for targeted biomedical applications. Such a study is also useful in understanding structural remodelling of silk debris that may be generated from silk-based implants. Ultrafine silk particles were prepared using a combination of efficient wet-milling and spray-drying processes with no addition of chemicals other than those used in degumming. Milling reduced the intermolecular stacking forces within the β-sheet crystallites without changing the intramolecular binding energy. Because of the rough morphology and the ultrafine size of the particles, degradation of silk particles by protease XIV was increased by about 3-fold compared to silk fibers. Upon biodegradation, the thermal degradation temperature of silk increased, which was attributed to the formation of tight aggregates by the hydrolyzed residual macromolecules. A model of the biodegradation mechanism of silk particles was developed based on the experimental data. The model explains the process of disintegration of β-sheets, supported by quantitative secondary structural analysis and microscopic images. © 2012 American Chemical Society.

History

Journal

Biomacromolecules

Volume

13

Issue

8

Pagination

2503 - 2512

Publisher

American Chemical Society

Location

Washington, D.C.

ISSN

1525-7797

eISSN

1526-4602

Language

eng

Publication classification

C1 Refereed article in a scholarly journal