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Studies on genomic DNA topology and stability in brain regions of Parkinson's disease
journal contribution
posted on 2006-05-15, 00:00 authored by M L Hegde, Veer GuptaVeer Gupta, M Anitha, T Harikrishna, S K Shankar, U Muthane, K Subba Rao, K S Jagannatha RaoDNA damage has been postulated as a mechanism of neuronal death in Parkinson's disease (PD). In the present study, genomic DNA was isolated from eight brain regions (frontal, temporal, and occipital cortex, hippocampus, caudate/putamen, thalamus, cerebellum, and midbrain) from five neuropathologically confirmed cases of Parkinson's disease and six control brains and analyzed for the presence of single and double strand breaks, melting temperature, EtBr intercalation, DNAse digestion pattern, and DNA conformations. The results showed that DNA from midbrain in PD accumulated significantly higher number of strand breaks than age-matched controls. Caudate nucleus/putamen, thalamus, and hippocampus also showed more DNA fragmentation compared to control brains. Circular dichroism studies showed that DNA conformation was altered with imprecise base stacking in midbrain, caudate nucleus/putamen, thalamus, and hippocampus in PD. However, DNA from frontal, temporal, and occipital cortex, and cerebellum was not affected significantly in PD group as compared to controls. This study provides a comprehensive database on stability, damage, and conformations of DNA in different regions in brains of PD patients. © 2006 Elsevier Inc. All rights reserved.
History
Journal
Archives of Biochemistry and BiophysicsVolume
449Issue
1-2Pagination
143 - 156Publisher DOI
ISSN
0003-9861eISSN
1096-0384Publication classification
C1.1 Refereed article in a scholarly journalUsage metrics
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No categories selectedKeywords
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiophysicsParkinson's diseaseDNA fragmentationDNA stabilityDNA conformationapoptosis\/necrosisstrand breaksmidbrainHUMAN SUBSTANTIA-NIGRALEWY BODY DISEASEMITOCHONDRIAL-DNACELL-DEATHDEOXYRIBONUCLEIC-ACIDDOPAMINERGIC-NEURONSALZHEIMERS-DISEASEOXIDATIVE DAMAGEIRON CONTENTNUCLEAR-DNA
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