munro-studiestoward-2013.pdf (5.51 MB)
Download file

Studies toward bivalent κ opioids derived from salvinorin A: heteromethylation of the furan ring reduces affinity

Download (5.51 MB)
journal contribution
posted on 2013-12-20, 00:00 authored by Thomas MunroThomas Munro, W Xu, D M Ho, L-Y Liu-Chen, B M Cohen
The recent crystal structure of the κ-opioid receptor (κ-OR) revealed, unexpectedly, that the antagonist JDTic is a bivalent ligand: in addition to the orthosteric pocket occupied by morphinans, JDTic also occupies a distinct (allotopic) pocket. Mutagenesis data suggest that salvinorin A (1) also binds to this allotopic pocket, adjacent to the aspartate residue that anchors the basic nitrogen atom of classical opiates (Asp138). It has been suggested that an H-bond donor appended to 1 might interact with Asp138, increasing affinity. Such a bivalent ligand might also possess altered functional selectivity. Based on modeling and known N-furanylmethyl opioid antagonists, we appended H-bond donors to the furan ring of 1. (Dimethylamino)methyl groups at C-15 or C-16 abolished affinity for κ-OR. Hydroxymethylation at C-16 was tolerated, but 15,16-bis-hydroxymethylation was not. Since allosteric modulators may go undetected in binding assays, we also tested these and other low-affinity derivatives of 1 for allosteric modulation of dynorphin A in the [(35)S]GTPγS assay. No modulation was detected. As an alternative attachment point for bivalent derivatives, we prepared the 2-(hydroxyethoxy)methyl ether, which retained high affinity for κ-OR. We discuss alternative design strategies for linked, fused or merged bivalent derivatives of 1.



Beilstein journal of organic chemistry




2916 - 2924




Frankfurt, Germany





Publication classification

C Journal article; C1.1 Refereed article in a scholarly journal

Copyright notice

2013, Munro et al