File(s) under permanent embargo
Synchrotron-FTIR microspectroscopy enables the distinction of lipid accumulation in thraustochytrid strains through analysis of individual live cells
journal contribution
posted on 2015-02-01, 00:00 authored by Jitraporn Vongsvivut, P Heraud, Adarsha Gupta, Tamilselvi Thyagarajan, Munish Puri, D McNaughton, Colin BarrowColin BarrowThe superior characteristics of high photon flux and diffraction-limited spatial resolution achieved by synchrotron-FTIR microspectroscopy allowed molecular characterization of individual live thraustochytrids. Principal component analysis revealed distinct separation of the single live cell spectra into their corresponding strains, comprised of new Australasian thraustochytrids (AMCQS5-5 and S7) and standard cultures (AH-2 and S31). Unsupervised hierarchical cluster analysis (UHCA) indicated close similarities between S7 and AH-7 strains, with AMCQS5-5 being distinctly different. UHCA correlation conformed well to the fatty acid profiles, indicating the type of fatty acids as a critical factor in chemotaxonomic discrimination of these thraustochytrids and also revealing the distinctively high polyunsaturated fatty acid content as key identity of AMCQS5-5. Partial least squares discriminant analysis using cross-validation approach between two replicate datasets was demonstrated to be a powerful classification method leading to models of high robustness and 100% predictive accuracy for strain identification. The results emphasized the exceptional S-FTIR capability to perform real-time in vivo measurement of single live cells directly within their original medium, providing unique information on cell variability among the population of each isolate and evidence of spontaneous lipid peroxidation that could lead to deeper understanding of lipid production and oxidation in thraustochytrids for single-cell oil development.
History
Journal
ProtistVolume
166Issue
1Pagination
106 - 121Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
1434-4610eISSN
1618-0941Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, Elsevier GmbHUsage metrics
Categories
No categories selectedKeywords
Thraustochytridslipidsmultivariate data analysis.polyunsaturated fatty acidssynchrotron Fourier transform infrared (S-FTIR) microspectroscopyMultivariate data analysisScience & TechnologyLife Sciences & BiomedicineMicrobiologyFATTY-ACID-COMPOSITIONINFRARED-SPECTROSCOPYFOURIEROMEGA-3IDENTIFICATIONPEROXIDATION
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC