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The ADAMTS5 metzincin regulates zebrafish somite differentiation

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journal contribution
posted on 2024-06-18, 07:29 authored by CM Dancevic, Y Gibert, J Berger, AD Smith, Clifford LiongueClifford Liongue, N Stupka, Alister WardAlister Ward, DR McCulloch
The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.

History

Journal

International Journal of Molecular Sciences

Volume

19

Article number

ARTN 766

Pagination

1 - 12

Location

Switzerland

Open access

  • Yes

ISSN

1661-6596

eISSN

1422-0067

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, the authors

Issue

3

Publisher

MDPI