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The Application of Sensitive Nano-Confined Nanoparticle System Using siRNA Targeting Extracellular Signal-Regulated Kinase (ERK)/Mitogen-Activated Protein Kinase (MAPK) Pathway in Gastrointestinal Cancer

journal contribution
posted on 2023-04-04, 00:11 authored by Runda Wu, Shan Tong, Mohammad KahriziMohammad Kahrizi, Zheng Zhu, Xin Chen
In this study we tried to develop a Sensitive Nano-Confined Nanoparticles (S-NCN) system using siRNA against Programmed death-ligand 1 (PDL-1) and Polo Like Kinase 1 (Plk1) to treat gastrointestinal cancer. In this regard, we first synthesized the corresponding materials, prepared the S-NCN system, and verified its functionality. Subsequently, we demonstrated in vitro that S-NCN delivery of siPlk1 could effectively downregulate the expression of Plk1 gene in GC1436 cells and lead to significant apoptosis of tumor cells. Xenografted gastrointestinal cancer model mice were used to evaluate the in vivo efficacy of the nanoparticle. We have demonstrated that the developed S-NCN system can efficiently bind siRNA and deliver it into target tumor cells, solving the main obstacle of siRNA delivery in vivo and effectively silencing target genes with a very potential delivery option.

History

Journal

Journal of Biomedical Nanotechnology

Volume

18

Pagination

986-1000

Location

United States

ISSN

1550-7033

eISSN

1550-7041

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Issue

4

Publisher

American Scientific Publishers