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The COMPASS subunit Spp1 links histone methylation to initiation of meiotic recombination

journal contribution
posted on 2013-01-01, 00:00 authored by L Acquaviva, L Szekvolgyi, Bernhard DichtlBernhard Dichtl, Beatriz Dichtl, L De, A Nicolas, V Geli
During meiosis, combinatorial associations of genetic traits arise from homologous recombination between parental chromosomes. Histone H3 lysine 4 trimethylation marks meiotic recombination hotspots in yeast and mammals, but how this ubiquitous chromatin modification relates to the initiation of double-strand breaks (DSBs) dependent on Spo11 remains unknown. Here, we show that the tethering of a PHD-containing protein, Spp1 (a component of the COMPASS complex), to recombinationally cold regions is sufficient to induce DSB formation. Furthermore, we found that Spp1 physically interacts with Mer2, a key protein of the differentiated chromosomal axis required for DSB formation. Thus, by interacting with H3K4me3 and Mer2, Spp1 promotes recruitment of potential meiotic DSB sites to the chromosomal axis, allowing Spo11 cleavage at nearby nucleosome-depleted regions.

History

Journal

Science

Volume

339

Issue

6116

Pagination

215 - 218

Publisher

American Association for the Advancement of Science

Location

Washington, D.C.

ISSN

0036-8075

eISSN

1095-9203

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2013, American Association for the Advancement of Science