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The Peri/postnatal Epigenetic Twins Study (PETS)
journal contribution
posted on 2013-02-01, 00:00 authored by Yuk Jing Loke, Boris Novakovic, Miina Ollikainen, Euan M Wallace, Mark P Umstad, Michael Permezel, Ruth Morley, Anne-Louise Ponsonby, Lavinia Gordon, John C Galati, Richard Saffery, Jeffrey CraigJeffrey CraigThe Peri/postnatal Epigenetic Twins Study (PETS) is a longitudinal cohort of 250 pairs of Australian twins and their mothers, who were recruited mid-way through pregnancy from January 2007 to September 2009. The study is centered on the developmental origins of health and disease paradigm (DOHaD) in which an adverse intrauterine environment predisposes the individual to complex disease in later life by reducing growth in utero and adversely altering developmental plasticity. Data concerning diet and lifestyle were collected from mothers during pregnancy, and samples of plasma and serum taken at 28 weeks' gestation. We attended 75% of all births, at which time we collected multiple biological samples including placenta, cord blood, and neonatal cheek cells, the latter from 91% of pairs. Chorionicity was recorded and zygosity was determined by DNA testing where necessary. Approximately 40% of the twins are monozygotic, two-thirds of which are dichorionic. Twins were seen again at 18 months of age and repeat blood and cheek swabs taken where possible. Studies of gene expression and the epigenetic marks of DNA methylation have so far revealed that twins exhibit a wide range of epigenetic discordance at birth, that one-third of the epigenome changes significantly between birth and 18 months; shared (maternal) environment, genetic factors, and non-shared intrauterine environment contribute to an increasing proportion of epigenetic variation at birth, respectively, and affect tissues differently, and that within-pair birth weight discordance correlates with epigenetic discordance in genes associated with lipid metabolism, supporting an epigenetic mechanism for DOHaD.
History
Journal
Twin research and human geneticsVolume
16Issue
1Pagination
13 - 20Publisher
Cambridge University PressLocation
Cambridge, Eng.Publisher DOI
ISSN
1832-4274Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2012, The AuthorsUsage metrics
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Categories
Keywords
DNA MethylationDietEpigenesis, GeneticFemaleFetal Growth RetardationGestational AgeHumansInfantInfant, NewbornLife StyleLongitudinal StudiesPregnancyTwinsScience & TechnologyLife Sciences & BiomedicineGenetics & HeredityObstetrics & Gynecologyepigeneticbirthdevelopmental originsenvironmentALLERGEN-SPECIFIC IGG1IMMUNE-RESPONSESGENETIC-FACTORSPREDICTIONCHORIONICITYPREGNANCIESEXPRESSIONEXPOSUREFAMINE