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The acetate regulatory gene facB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator.

Version 2 2024-06-03, 10:34
Version 1 2017-07-27, 13:34
journal contribution
posted on 2024-06-03, 10:34 authored by RB Todd, RL Murphy, HM Martin, Julie SharpJulie Sharp, MA Davis, ME Katz, MJ Hynes
Genetic studies have indicated that the facB gene of Aspergillus nidulans is a major regulatory gene involved in acetamide and acetate utilisation. Sequencing of the facB gene revealed that it encodes a protein that contains an N-terminal GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster for DNA binding, leucine zipper-like heptad repeat motifs and central and C-terminal acidic alpha-helical regions, consistent with a function as a DNA-binding transcriptional activator. The Zn(II)2Cys6 cluster shows strong similarity with those of the Saccharomyces cerevisiae carbon metabolism regulatory proteins CAT8 and SIP4. A significant level of similarity with CAT8 is found throughout the length of the protein, suggesting at least partial functional homology. The facB genes of Aspergillus oryzae and Aspergillus niger were also sequenced and found to be highly conserved. Deletion of the facB gene confirmed that it is required for growth on acetate as a sole carbon source. Functional dissection using deletion and fusion constructs and in vitro mutagenesis indicated that the Zn(II)2Cys6 cluster and the C-terminal end of the protein are required for function.

History

Journal

Molecular and General Genetics MGG

Volume

254

Pagination

495-504

Location

Germany

ISSN

0026-8925

Language

eng

Publication classification

CN.1 Other journal article

Issue

5

Publisher

Springer Verlag