Deakin University
Browse

File(s) under permanent embargo

The development of genetic tools for dissecting the biology of malaria parasites

Version 2 2024-06-03, 12:27
Version 1 2017-07-24, 08:33
journal contribution
posted on 2024-06-03, 12:27 authored by Tania De Koning-WardTania De Koning-Ward, CJ Janse, AP Waters
Plasmodium parasites are haploid unicellular organisms that cause malaria. In the last decade, transfection systems have been developed for both human and animal model species of Plasmodium, providing a broad range of genetic tools for the study of malaria parasite biology. Transient transfection has been used to provide insight into the regulation of gene expression by Plasmodium spp. The development of stable transfection technologies has provided the opportunity to express transgenes in Plasmodium spp., as well as elucidate the function of proteins by disrupting, modifying, or replacing the genes encoding them. These genetic tools represent an important breakthrough for malaria research and will significantly contribute to our understanding of the biology of the parasite. However, further developments in this technology are still required, especially because the full genome sequence of the major human malaria parasite Plasmodium falciparum will shortly be available. Ultimately, the biological information obtained through genetic manipulation of Plasmodium spp. will facilitate a more rational approach to vaccine and drug design.

History

Journal

Annual review of microbiology

Volume

54

Pagination

157-185

Location

Palo Alto, Calif.

ISSN

0066-4227

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2000, Annual Reviews

Publisher

Annual Reviews

Usage metrics

    Research Publications

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC