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The distribution of CpG islands in mammalian chromosomes
journal contribution
posted on 1994-07-01, 00:00 authored by Jeffrey CraigJeffrey Craig, W A BickmoreUsing fluorescent in situ suppression hybridization to metaphase chromosomes, we have directly shown that CpG islands are predominantly found in the early replicating (R band) regions of the genome. Conversely, late replicating (G band) DNA is sparsely populated with islands. The very highest concentration of CpG islands is in a subset of R bands, most of which are known as T bands. We suggest that there is an interdependence between the differences in island density and the behaviour of chromosomal domains. Our findings indicate which regions of the genome will yield the highest density of coding sequence information. An awareness of local island density may influence the choice of method for identifying exons in genomic DNA.
History
Journal
Nature geneticsVolume
7Issue
3Pagination
376 - 382Publisher
Nature Publishing GroupLocation
London, Eng.Publisher DOI
ISSN
1061-4036Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1994, Nature Publishing GroupUsage metrics
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No categories selectedKeywords
AnimalsBase SequenceCells, CulturedChromosome BandingChromosome MappingChromosomes, HumanDNA ReplicationDNA Restriction EnzymesElectrophoresis, Gel, Pulsed-FieldFemaleGenesGenetic MarkersGenome, HumanHumansIn Situ Hybridization, FluorescenceLymphocytesMaleMammalsMethylationMolecular Sequence DataPolymorphism, GeneticRepetitive Sequences, Nucleic AcidScience & TechnologyLife Sciences & BiomedicineGenetics & HeredityGENOME ORGANIZATIONDNA-REPLICATIONGENE-EXPRESSIONX-CHROMOSOMEBANDSCHROMATINMOUSENUCLEOSKELETONNONUNIFORMEVOLUTION
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