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The effect of climatic simulations on DNA persistence on glass, cotton and polyester

journal contribution
posted on 2019-01-01, 00:00 authored by C Chen, A Pistono, S Ryan, Bianca SzkutaBianca Szkuta, G E Meakin
It is important to understand the variables impacting DNA persistence when considering the recovery, and evaluative interpretation, of DNA evidence from crime scenes. Whilst it is known that temperature, humidity and UV affect DNA persistence, little research has been conducted to explore these effects in a combined and controlled manner. This study includes two experiments in which a climate chamber was used to simulate climatic conditions over a repeating 24-h period. Aliquots of ∼50 ng DNA were added to each substrate and DNA recovered at 0, 1, 3 and 7 days after deposition. Samples were run in triplicate, extracted and quantified. The first experiment investigated the effect of typical Southern English winter and summer days on DNA persistence on glass and cotton, with DNA being recovered by wet and dry swabs from glass and mini-tapes from cotton. The second experiment investigated the effect of typical Northern Italian winter and summer days on DNA persistence on cotton and polyester, with DNA being recovered by wet and moist swabs from both fabrics. Quantities of DNA on all substrates significantly declined over 7 days under summer conditions (p < 0.05), and more DNA tended to persist on the fabric substrates in both studies under conditions of winter than summer. These results contribute to our understanding of DNA persistence under different climatic conditions and will help inform investigators’ DNA recovery strategies.

History

Journal

Forensic science international: genetics supplement series

Volume

7

Issue

1

Pagination

274 - 276

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

1875-1768

eISSN

1875-175X

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

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