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The effect of water deprivation on the expression of atrial natriuretic pepide and its receptors in the spinifex hopping mouse, Notomys alexis

Version 2 2024-06-17, 03:57
Version 1 2014-10-27, 16:27
journal contribution
posted on 2024-06-17, 03:57 authored by R Heimeier, B Davis, John Donald
This study investigated the mRNA expression of the atrial natriuretic peptide (ANP) system (peptide and receptors) during water deprivation in the spinifex hopping mouse, Notomys alexis, a native of central and western Australia that is well adapted to survive in arid environments. Initially, ANP, NPR-A and NPR-C cDNAs (partial for receptors) were cloned and sequenced, and were shown to have high homology with those of rat and mouse. Using a semi-quantitative multiplex PCR technique, the expression of cardiac ANP mRNA and renal ANP, NPR-A, and NPR-C mRNA was determined in 7- and 14-day water-deprived hopping mice, in parallel with control mice (access to water). The levels of ANP mRNA expression in the heart remained unchanged, but in the kidney ANP mRNA levels were increased in the 7-day water-deprived mice, and were significantly decreased in the 14-day water-deprived mice. NPR-A mRNA levels were significantly higher in 7-day water-deprived mice while no change for NPR-A mRNA expression was observed in 14-day water-deprived mice. No variation in NPR-C mRNA levels was observed. This study shows that water deprivation differentially affects the expression of the ANP system, and that renal ANP expression is more important than cardiac ANP in the physiological adjustment to water deprivation.

History

Journal

Comparative biochemistry and physiology - part A molecular & integrative physiology

Volume

132

Pagination

893-903

Location

New York, N.Y.

ISSN

1095-6433

eISSN

1531-4332

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2002, Elsevier Science Inc.

Issue

4

Publisher

Elsevier Science Inc.