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The effects of processing and mastication on almond lipid bioaccessibility using novel methods of in vitro digestion modelling and micro-structural analysis
journal contributionposted on 2023-03-08, 03:04 authored by Giuseppina Mandalari, Myriam M-L Grundy, Terri Grassby, Mary L Parker, Kathryn L Cross, Simona Chessa, Carlo Bisignano, Davide Barreca, Ersilia Bellocco, Giuseppina Lagana, Peter J Butterworth, Richard M Faulks, Peter J Wilde, Peter R Ellis, Keith W Waldron
A number of studies have demonstrated that consuming almonds increases satiety but does not result in weight gain, despite their high energy and lipid content. To understand the mechanism of almond digestion, in the present study, we investigated the bioaccessibility of lipids from masticated almonds during in vitro simulated human digestion, and determined the associated changes in cell-wall composition and cellular microstructure. The influence of processing on lipid release was assessed by using natural raw almonds (NA) and roasted almonds (RA). Masticated samples from four healthy adults (two females, two males) were exposed to a dynamic gastric model of digestion followed by simulated duodenal digestion. Between 7·8 and 11·1 % of the total lipid was released as a result of mastication, with no significant differences between the NA and RA samples. Significant digestion occurred during the in vitro gastric phase (16·4 and 15·9 %) and the in vitro duodenal phase (32·2 and 32·7 %) for the NA and RA samples, respectively. Roasting produced a smaller average particle size distribution post-mastication; however, this was not significant in terms of lipid release. Light microscopy showed major changes that occurred in the distribution of lipid in all cells after the roasting process. Further changes were observed in the surface cells of almond fragments and in fractured cells after exposure to the duodenal environment. Almond cell walls prevented lipid release from intact cells, providing a mechanism for incomplete nutrient absorption in the gut. The composition of almond cell walls was not affected by processing or simulated digestion.
JournalBRITISH JOURNAL OF NUTRITION
PublisherCAMBRIDGE UNIV PRESS
Science & TechnologyLife Sciences & BiomedicineNutrition & DieteticsAlmondsLipid bioaccessibilityMasticationCell wallsProcessingTYPE-2 DIABETES-MELLITUSBODY-WEIGHTSEEDSCONSUMPTIONAPPETITEDIETSAdultBiological AvailabilityCell WallDigestionDuodenumFemaleFood HandlingGastric MucosaHot TemperatureHumansIn Vitro TechniquesLipidsMaleModels, BiologicalNutsParticle SizePrunusNutrition1 Underpinning research1.1 Normal biological development and functioningOral and gastrointestinalMetabolic and endocrineFood Sciences not elsewhere classifiedAnimal Production not elsewhere classifiedNutrition and Dietetics not elsewhere classified