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Download fileThe identification of mitochondrial DNA variants in glioblastoma multiforme
journal contribution
posted on 2014-01-02, 00:00 authored by Ka Yu Yeung, Adam Dickinson, Jacqueline F Donoghue, Galina Polekhina, Stefan J White, Dimitris K Grammatopoulos, Matthew McKenzieMatthew McKenzie, Terrance G Johns, Justin C St JohnBACKGROUND: Mitochondrial DNA (mtDNA) encodes key proteins of the electron transfer chain (ETC), which produces ATP through oxidative phosphorylation (OXPHOS) and is essential for cells to perform specialised functions. Tumor-initiating cells use aerobic glycolysis, a combination of glycolysis and low levels of OXPHOS, to promote rapid cell proliferation and tumor growth. Glioblastoma multiforme (GBM) is an aggressively malignant brain tumor and mitochondria have been proposed to play a vital role in GBM tumorigenesis. RESULTS: Using next generation sequencing and high resolution melt analysis, we identified a large number of mtDNA variants within coding and non-coding regions of GBM cell lines and predicted their disease-causing potential through in silico modeling. The frequency of variants was greatest in the D-loop and origin of light strand replication in non-coding regions. ND6 was the most susceptible coding gene to mutation whilst ND4 had the highest frequency of mutation. Both genes encode subunits of complex I of the ETC. These variants were not detected in unaffected brain samples and many have not been previously reported. Depletion of HSR-GBM1 cells to varying degrees of their mtDNA followed by transplantation into immunedeficient mice resulted in the repopulation of the same variants during tumorigenesis. Likewise, de novo variants identified in other GBM cell lines were also incorporated. Nevertheless, ND4 and ND6 were still the most affected genes. We confirmed the presence of these variants in high grade gliomas. CONCLUSIONS: These novel variants contribute to GBM by rendering the ETC. partially dysfunctional. This restricts metabolism to anaerobic glycolysis and promotes cell proliferation.
History
Journal
Acta neuropathologica communicationsVolume
2Issue
1Pagination
1 - 21Publisher
BioMed CentralLocation
London, EnglandPublisher DOI
Link to full text
eISSN
2051-5960Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2014, Yeung et alUsage metrics
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Categories
Keywords
AnimalsAntimetabolitesBrainBrain NeoplasmsCell Line, TumorCell Transformation, NeoplasticDNA, MitochondrialGene Expression Regulation, NeoplasticGenetic VariationGlioblastomaGlycolysisHeterograftsHigh-Throughput Nucleotide SequencingHumansMiceMitochondrial Proton-Translocating ATPasesModels, MolecularNeural Stem CellsOxidative PhosphorylationZalcitabineScience & TechnologyLife Sciences & BiomedicineNeurosciencesNeurosciences & NeurologyMitochondrial DNATumorigenesisGenetic variantsAerobic glycolysisDepletionSOMATIC MUTATIONSCOPY NUMBERNUCLEARGENOMECANCERINVOLVEMENTSEQUENCEDISEASEFAMILYMTDNA