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The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding

Version 3 2024-06-18, 19:32
Version 2 2024-06-06, 07:39
Version 1 2020-03-09, 10:27
journal contribution
posted on 2024-06-18, 19:32 authored by SS Ch’ng, Adam WalkerAdam Walker, M McCarthy, TK Le, N Thomas, A Gibbons, M Udawela, S Kusljic, B Dean, A Gogos
Ovarian hormones, such as estrogens and progesterone, are known to exert beneficial effects on cognition and some psychiatric disorders. The basis of these effects is not fully understood, but may involve altered cholinergic neurotransmission. This study aimed to investigate how a lack of ovarian hormones would impact muscarinic receptor-induced deficits in prepulse inhibition (PPI) and muscarinic receptor density in several brain regions. Adult female rats were either ovariectomized, to remove the source of ovarian hormones, or left intact (sham-operated). PPI is a measure of sensorimotor gating that is typically impaired in schizophrenia patients, and similar deficits can be induced in rats by administering scopolamine, a muscarinic receptor antagonist. Our results revealed no significant effects of ovariectomy on PPI after saline or scopolamine treatment. Autoradiography was performed to measure cholinergic muscarinic receptor binding density using [3H]-pirenzepine, [3H]-AF-DX, and [3H]-4-DAMP, to label M1, M2/M4, and M3 receptors, respectively. We examined the amygdala, caudate putamen, dorsal hippocampus, motor cortex, retrosplenial cortex, and ventromedial hypothalamus. There were no significant group differences in any region for any muscarinic receptor type. These results suggest that removing peripheral ovarian hormones does not influence the cholinergic muscarinic receptor system in the context of PPI or receptor binding density.

History

Journal

Brain Sciences

Volume

10

Article number

ARTN 106

Location

Switzerland

Open access

  • Yes

ISSN

2076-3425

eISSN

2076-3425

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2020, The Authors

Issue

2

Publisher

MDPI