Deakin University
Browse

File(s) not publicly available

The induction of α-lactalbumin in rat mammary explants in the absence of exogenous prolactin: Effects of progesterone and estrogen

journal contribution
posted on 1981-01-01, 00:00 authored by Kevin Nicholas, L Sankaran, Y J Topper
Mammary explants from both pregnant and virgin rats cultured in medium containing insulin, hydrocortisone and prolactin show a progressive increase in α-lactalbumin activity. However, when cultured with insulin and hydrocortisone only, explants from pregnant, but not those from virgin rats show an induction of α-lactalbumin-like activity similar to that seen when prolactin is present. The activity induced in the absence of exogenous prolactin corresponds to newly synthesized α-lactalbumin molecules, since 1) the activity is suppressed completely by rabbit anti-rat α-lactalbumin serum and 2) culture in the presence of [3H]-amino acids generates [3 H] - α-lactalbumin, identified by SDS-polyacrylamide gel electrophoresis. Mammary tissue from virgin rats can be rendered responsive to insulin and hydrocortisone in this regard by administration in vivo of either progesterone or estrogen, but not by desoxycorticosterone acetate. This ability to convert virgin rat mammary cells to a state in which they are independent of exogenous prolactin in terms of α-lactalbumin synthesis represents a heretofore unreported effect of the female sex steroids. © 1981 by The Endocrine Society.

History

Journal

Endocrinology

Volume

109

Issue

3

Pagination

978 - 980

ISSN

0013-7227

eISSN

1945-7170

Usage metrics

    Research Publications

    Categories

    No categories selected

    Keywords

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC